Journal: Kidney Research and Clinical Practice

Article Title: Pan-Nox inhibitor treatment improves renal function in aging murine diabetic kidneys

doi: 10.23876/j.krcp.23.004

Figure Lengend Snippet: F4/80, F4/80 stain; PAI-1, plasminogen activator inhibitor-1 stain; PAS, periodic acid-Schiff stain; STZ, streptozotocin; TGF-β1, transforming growth factor beta-1 stain. Original magnification, ×400.

Article Snippet: Slides were then incubated overnight with rabbit polyclonal anti-TGF-β1 antibodies (1:200; Santa Cruz Biotechnology Inc.) at 4 °C, rabbit polyclonal anti-PAI-1 antibodies (1:60; American Diagnostica) or mouse monoclonal anti-F4/80 antibodies (1:100; Serotec Inc.).

Techniques: Staining

Journal: Kidney Research and Clinical Practice

Article Title: Pan-Nox inhibitor treatment improves renal function in aging murine diabetic kidneys

doi: 10.23876/j.krcp.23.004

Figure Lengend Snippet: (A) Mesangial expansion score, (B) scoring for F4/80-positive cells, (C) IHC staining score for plasminogen activator inhibitor 1 (PAI-1), and (D) IHC staining score for transforming growth factor beta 1 (TGF-β1). Data are shown as the mean ± standard error of mean. p < 0.001 vs. control or APX-115, # p < 0.05, ## p < 0.01, ### p < 0.001 vs. streptozotocin (STZ).

Article Snippet: Slides were then incubated overnight with rabbit polyclonal anti-TGF-β1 antibodies (1:200; Santa Cruz Biotechnology Inc.) at 4 °C, rabbit polyclonal anti-PAI-1 antibodies (1:60; American Diagnostica) or mouse monoclonal anti-F4/80 antibodies (1:100; Serotec Inc.).

Techniques: Immunohistochemistry, Control

Journal: Kidney Research and Clinical Practice

Article Title: Pan-Nox inhibitor treatment improves renal function in aging murine diabetic kidneys

doi: 10.23876/j.krcp.23.004

Figure Lengend Snippet: (A) Representative immunoblots for transforming growth factor beta 1 (TGF-β1) and monocyte chemoattractant protein 1 (MCP-1); (B) densitometric analysis of immunoblots for TGF-β1 and MCP-1; (C) representative immunoblots for Nox1, Nox2, and Nox4; and (D) densitometric analysis of immunoblots for Nox1, Nox2, and Nox4. Data are shown as the mean ± standard error of mean. Nox, nicotinamide adenine dinucleotide phosphate oxidase; STX, streptozotocin. * p < 0.05, ** p < 0.01 vs. control or APX-115, # p < 0.05, ## p < 0.01 vs. STZ.

Article Snippet: Slides were then incubated overnight with rabbit polyclonal anti-TGF-β1 antibodies (1:200; Santa Cruz Biotechnology Inc.) at 4 °C, rabbit polyclonal anti-PAI-1 antibodies (1:60; American Diagnostica) or mouse monoclonal anti-F4/80 antibodies (1:100; Serotec Inc.).

Techniques: Western Blot, Control

Journal: Scientific Reports

Article Title: Selective activation of PPARα by pemafibrate mitigates peritoneal inflammation and fibrosis through suppression of NLRP3 inflammasome and modulation of inflammation

doi: 10.1038/s41598-024-74340-5

Figure Lengend Snippet: Pemafibrate ameliorated MGO-induced peritoneal fibrosis in mice. ( a-e ) Representative immunohistochemical analyses showing ( a ) α-SMA, ( b ) FSP-1, ( c ) collagen I, ( d ) collagen III, and ( e ) TGF-β1 expression in peritoneal tissue from control mice, MGO-injected mice, and MGO-injected mice with pemafibrate (scale bar, 100 μm). ( f-k ) Quantitative analysis of ( f ) α-SMA-positive cells, ( g ) FSP-1-positive cells, ( h ) collagen I-positive area, ( i ) collagen III-positive area, and ( j ) TGF-β1-positive cells from control mice, MGO-injected mice, and MGO-injected mice with pemafibrate. n = 5 per group. ( k ) TGF-β1 protein levels in mouse PD effluent were determined by ELISA. n = 5 per group. * indicates P < 0.05 by one-way ANOVA followed by Tukey’s post-hoc test. Pema, pemafibrate.

Article Snippet: The following primary antibodies were used: rabbit polyclonal anti-PPARα antibody (Abcam, Cambridge, UK, ab215270), mouse monoclonal anti-α-smooth muscle actin (anti-α-SMA) antibody (Sigma-Aldrich, St. Louis, MO, a2547), anti-fibroblast-specific protein 1 (anti-FSP-1) antibody (Abcam, ab41532), rabbit polyclonal anti-collagen I antibody (Abcam, ab34710), rabbit polyclonal anti-collagen III antibody (GeneTex, Irvine, CA, GTX102997), rabbit polyclonal anti-TGF-β1 antibody (Santa Cruz Biotechnology, Dallas, TX, sc-130348), mouse monoclonal anti-TNF-α antibody (Abcam, ab1793), mouse monoclonal anti-mouse CD68 antibody (Abcam, ab125212), anti-mouse CD163 antibody (Abcam, ab182422), and monoclonal anti-IFN-γ antibody (Abcam, ab267369).

Techniques: Immunohistochemical staining, Expressing, Control, Injection, Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Selective activation of PPARα by pemafibrate mitigates peritoneal inflammation and fibrosis through suppression of NLRP3 inflammasome and modulation of inflammation

doi: 10.1038/s41598-024-74340-5

Figure Lengend Snippet: Pemafibrate inhibited IFN-γ-induced fibrotic and inflammatory markers in HPMCs. ( a , b ) Representative western blotting analyses showing the expression levels of ( a ) TGF-β and ( b ) secreted fibronectin from control HPMCs, and HPMCs with or without pemafibrate (40 or 60 µmol/L) after 48 h of IFN-γ stimulation. ( c-h ) Representative western blotting analyses showing the expression levels of ( c ) phospho-NF-κB and NF-κB, ( d ) phospho-IκBα, ( e ) phospho-cJun (a component of AP-1), ( f ) phospho-STAT1, ( g ) CXCL9, and ( h ) CXCL10 from control HPMCs, and HPMCs with or without pemafibrate (40 or 60 µmol/L) after 24 h of IFN-γ stimulation. n = 5 per group. * indicates P < 0.05 by one-way ANOVA followed by Tukey’s post-hoc test. Pema, pemafibrate.

Article Snippet: The following primary antibodies were used: rabbit polyclonal anti-PPARα antibody (Abcam, Cambridge, UK, ab215270), mouse monoclonal anti-α-smooth muscle actin (anti-α-SMA) antibody (Sigma-Aldrich, St. Louis, MO, a2547), anti-fibroblast-specific protein 1 (anti-FSP-1) antibody (Abcam, ab41532), rabbit polyclonal anti-collagen I antibody (Abcam, ab34710), rabbit polyclonal anti-collagen III antibody (GeneTex, Irvine, CA, GTX102997), rabbit polyclonal anti-TGF-β1 antibody (Santa Cruz Biotechnology, Dallas, TX, sc-130348), mouse monoclonal anti-TNF-α antibody (Abcam, ab1793), mouse monoclonal anti-mouse CD68 antibody (Abcam, ab125212), anti-mouse CD163 antibody (Abcam, ab182422), and monoclonal anti-IFN-γ antibody (Abcam, ab267369).

Techniques: Western Blot, Expressing, Control